Exosome culture workstation
The exosome culture workstation is an intelligent equipment designed for large-scale and efficient automatic production of mesenchymal stem cell exosomes.
Description
Product Introduction
- Reagent storage area
Keep the bag medium at 2~8°C to prevent the performance degradation caused by repeated prewarming of the reagent during artificial culture.
- Human-computer interaction area
The culture process can be edited and run, and the environmental data in the culture process can be displayed and saved, and the operation interface is simple and easy to use.
- Cell culture area
The incubator provides a suitable cell culture environment, and maintains the set temperature and carbon dioxide concentration inside to realize the automatic culture of cells; The table is fixed inside the incubator and rocked according to the program to accelerate cell growth and promote exosome harvesting.
Product Advantages:
- automation
Equipped with a single-use, fully enclosed tubing system with pre-packaged sheet microcarriers, the whole process is automatically processed from cell culture to exosome production, reducing external environmental requirements and saving costs. The system automatically records the whole process operation steps, provides more accurate and consistent culture conditions, and provides a more intelligent and large-scale culture program for the production of exosomes in small and medium-sized systems. Provide mature and complete production process and standardized production process. A set of standardized production processes can be quickly replicated in multiple laboratories of users, helping users quickly form their own product systems.
- Scale
The system automatically records the whole process operation steps, provides more accurate and consistent culture conditions, and provides a more intelligent and large-scale culture program for the production of exosomes in small and medium-sized systems.
- standardization
Provide mature and complete production process and standardized production process. A set of standardized production processes can be quickly replicated in multiple laboratories of users, helping users quickly form their own product systems.
Product performance
1. Exosome concentration and particle size
| Sampling time point | Exosome concentration (unit/mL) | Average particle size (nm) |
| 3 Days | 4.74E+09 | 90.9 |
| 6 Days | 5.93E+09 | 103.2 |
| 9 Days | 4.24E+09 | 135.9 |
| 12 Days | 4.22E+09 | 139.5 |
| 15 Days | 4.30E+09 | 139.9 |
*Exosome counting instrument: NTA
Day 3
Day 6
Day 15
Day 12
Day 15
2. Harvesting the total amount of exosomes
One culture cycle lasts 15 days, requiring 5L of culture medium. After one cycle every 3 days, 1L of supernatant can be harvested, and a total of 2.34E+13 exosomes can be harvested.
| Sampling time point | Exosome concentration (unit/mL) | Number of exosomes harvested | Culture medium volume (mL) |
| 3 Days | 4.74E+09 | 4.74E+12 | 1000 |
| 6 Days | 5.93E+09 | 5.93E+12 | 1000 |
| 9 Days | 4.24E+09 | 4.24E+12 | 1000 |
| 12 Days | 4.22E+09 | 4.22E+12 | 1000 |
| 15 Days | 4.30E+09 | 4.30E+12 | 1000 |
| Total | 2.34E+13 | 5000 | |
* Calculation of total amount of harvested exosomes
3. Exosome markers protein
Day 3
Day 6
Day 9
Day 12
Day 15
4. Protein marker detect by Western blot
Negative: Calnexin
Positive: CD9、CD63、TSG101
Negative: Calnexin
Positive: CD9
Positive: CD63
Positive: TSG101
Newly upgraded formula, with defined chemical composition and perfectly combines cell isolation, subculture and exosome harvesting.
