Gentle Digestive Enzymes For Stem Cells
- Artificial recombinant system, without human or animal derived components.
- Designed for passage and digestion of mesenchymal stem cells,
- Gentle and less harmful to cells, limit digestion time is up to 15 min.
Description
This animal-derived free digestive enzymes specifically designed for passage and digestion of mesenchymal stem cells, gentle and less harmful to cells, ensure the cell viability of stem cells above 90% after 15 minutes of infiltration in digestive enzymes, thus solving the difficulty in digestion time and digestion strength control of trypsin digested cells. After digestion, only dilution of culture supernatant is required without termination, so this product is better suitable for large-scale cell production. This product is free of any animal-derived components with defined components, greatly reducing the workload and difficulty in declaration of stem cell drugs.
| Product name | Item No. | Product specification | Purpose |
| Gentle digestive enzymes for stem cells | NC1004.1 | 500 mL/bottle | Specially designed for digestion and passage of mesenchymal stem cells |
| NC1004.2 | 100 mL/bottle |
- Artificial recombinant system, without human or animal derived components, and the endotoxin in the products can be controlled at <0.25EU/mL. It has been filed with the FDA DMF, with the filing No. of 37790, which greatly reduces the workload and dif ficulty in submitting the application f or stem cell drugs.
- The conventional digestion time is 2 min, and the limit digestion time is up to 15 min, which has solved the problem that the digestion time and digestion strength are difficult to control when the cells are digested by traditional trypsin.
- After digestion, there is no need to stop. The product can be obtained by diluting the supernatant of cell culture, which can greatly reduce the use cost.
Product Advantages
Free of serum and animal-derived components
This product is an animal-derived gene-free recombinant digestive enzymes, without residual exogenous protein and virus.
Gentle digestion
Normal digestion lasts for 1-2 minutes, easy to control. High operational fault tolerance, suitable for large-scale production.
Higher protection for cells
During normal digestion, the cell viability is 10% higher than that of conventional pancreatic enzymes, and the cell recovery is 11%-14% higher than that of conventional pancreatic enzymes.
Extreme digestion lasts for up to15 minutes
Extreme digestion of cells lasts for up to 15 minutes almost without any effect, and cells still grow normally after subculture.
New-Generation Digestion Product
Comprehensive comparison of the new-generation digestion products (stem cell mild digestive enzyme) and traditional digestion product (porcine and bovine natural trypsin’s)
Type | Subtype | Traditional product | Stem Cell Mild Digestive Enzyme | |
Digestion performance | Normal digesti- on(without excessive digestion) | Cell viability | 83% | above 95% |
The cell count obtained by continuing to culture after digestion | 1.86×106 | 1.94×106 | ||
Digest overtime (excessive digestion) | Cell viability | 60% | above 95% | |
The cell count obtained by continuing to culture after digestion | 1.07×106 | 1.88×106 | ||
Extreme digest- ion(15-minute digestion) | Cell viability | 0-10% | above 90% | |
The cell count obtained by continuing to culture after digestion | Unable to harvest cells | 1.78×106 | ||
Operation convenience | Does it need to be terminated after digestion? | Required | No |
Does it need to be stored at -20°C? | Required | No | |
Is it ready-for-use? | No | Yes | |
Operation convenience | Cost of digestion termination | High. Serum, complete media or trypsin inhibitors are required | Digestion does not need to be terminated It can be used by being diluted with the culture supernatant |
Cost of virus test before cell application | High. Animal viruses such as swine foot-and-mouthdisease and bovine mad cow disease need to be tested | No test is required since there is no animal source |
Product safety | Is it free of animal-derived components? | No | Yes |
Is it possible to confirm that there is no animal virus? | No | Yes | |
Difficulty in the application of cell drugs | Confirmed difficulty affected by product residues | Extremely high Impurity components are unknown and difficult to prove | Low. The ingredients are clear, all of which are artificially synthesized components |
*The above data are the corresponding data on P4 cells obtained passaging P3 cells harvested after digesting the P2 seed cells inoculated with umbilical cord-derived MSCs in T25 culture fla sks at the seeding density of 8000 cells/cm2 after 72 hours.
Compared with traditional trypsin, digestive enzymes perform better
P2 MSC seed cells were subcultured for 72 hours and then digested with mild enzyme and traditional trypsin, respectively, to obtain P3 MSCs, and the cell viability after digestion was detected.
P3 MSC cells were further cultured for 72 hours, digested with mild enzyme and traditional trypsin, respectively, to harvest P4 MSC cells, and the cells were counted.
Type | Traditional trypsin | Mild digestive enzyme |
P3 MSC cell Viability | 85% | 95% |
P3 MSC cell count harvested | 2.06×106 cells | 2.28×106 cells |
P3 MSC cell Viability | 83% | 96% |
P4 MSC cell count harvested | 2.06×106 cells | 1.94×106 cells |
| Item | Sub-item | Conventional product | Gentle digestive enzymes for stem cells | ||||
| Digestive performance | Proper digestion (Without) | Cell viability | 83% | above 95% | |||
| Cells obtained by continuous culture after digestion | 1.86×10⁶ | 1.94×10⁶ | |||||
| Overtime digestion (With over-digestion) | Cell viability | 60% | above 95% | ||||
| Cells obtained by continuous culture after digestion | 1.07×10⁶ | 1.88×10⁶ | |||||
| Extreme digestion (15 min digestion) | Cell viability | 0-10% | above 90% | ||||
| Cells obtained by continuous culture after digestion | None | 1.78×10⁶ | |||||
| Convenience | Termination required after digestion? | Yes | No | ||||
| Kept at -20°C? | Yes | No | |||||
| Ready for pickup and use? | No | Yes | |||||
| Comprehensive cost of use | Cost of digestion termination | High. Serum, complete media, or trypsin inhibitors are required. | None. No digestion termination is required. It is enough to dilute with culture supernatant. | ||||
| Cost of virus test before cell application | High. Animal viruses such as swine foot-and-mouth disease and bovine mad cow disease need to be tested. | None. No test is required as it is free of animal-derived components. | |||||
| Product safety | Free of animal-derived components? | No | Yes | ||||
| Confirm the absence of animal viruses? | No | Yes | |||||
| Difficulty in declaration of cell drugs | Difficulty in demonstration of the effect of residual product | Extremely large. It is difficult to demonstrate because impurity components are unknown. | Low. The ingredients are clear, all of which are artificially synthesized components. | ||||
* The above data was the corresponding data obtained from P4 cells obtained by inoculating P2 seed cells of MSC of umbilical cord origin in T25 culture flasks with an inoculation density of 8,000 cells/cm² and subculturing the P3 cells obtained by digestion after 72 h.