MSC Serum-free Medium (GMP grade)
Description
MSC Serum-free Medium (GMP grade) Kit
| Product name | Item No. | Packing specifications | Storage condition | Shelf life |
| Serum-free minimal medium for mesenchymal stem cells (GMP grade) | NC0106 | 500 mL/bottle | 2-8°C, protected from light | 12 months |
| MSC serum free medium additive (GMP grade) | NC0106.S | 5 mL/bottle | -20°C, protected from light | 12 months |
Product Advantages
Industrial level cultivation
Improve cell adhesion performance, suitable for large-scale production of cell products and 3D culture systems.
Continuous passage
Primary separation and high passage continuous passage to P20 can be achieved without changing the culture medium.
Ingredient limitations
There are no more than 20 types of proteins, with a total content of less than 1.5mg/mL, all of which are artificial recombinant systems and have no human or animal sources
The inoculum size is provided below if petri dish of different surface area is used:
Culture flask/dish | Approximate bottom area (cm2) | Day 0 medium volume (mL) | Day 2 medium volume (mL) | Tissue segment added (number) |
60 mm dish | 21 | 2 | 3 | 5 |
100mm dish | 55 | 5 | 7 | 12 |
T-25 flask | 25 | 2 | 3 | 6 |
T-75 flask | 75 | 7 | 8 | 15 |
T-175 flask | 175 | 15 | 20 | 40 |
T-225 flask | 225 | 20 | 25 | 50 |
Cell Karyotype Detection
Chromosome coloring method: G banding | Banding standard:400-550 | Analyzing tool: CytoVision,Leica |
The number of detected cells:100 | Count the number of cells:100 | Karyotyping Cell Number:50 |
Test Result | ||
Karyotype analysis results of 100 dividing cells | ||
Chromosomal Analysis Project | Abnormal cell count | Reference |
Chromatids and chromosome breaks | 3 | ≤ 8 |
Abnormal structure | 1 | ≤ 2 |
Hyperdiploid | 0 | ≤ 2 |
Hypodiploid | 5 | ≤ 18 |
Polyploid | 0 | ≤ 4 |
Passage performance
GMP MSC Cell morphology
Phenotypic testing
